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Soil microbial fuel cells (SMFCs) function as bioelectrochemical energy harvesters that convert electrons stored in soil organic matter into useful electrical energy. Broadly, an SMFC comprises three essential components: an anode buried in the soil (the negative terminal), a colony of exoelectrogenic microorganisms residing on this anode, and a cathode (the positive terminal). As the exoelectrogens respire, they release electrons to the anode, which acts as an external receptor. These released electrons then flow through a load (e.g. a resistor), connecting the anode and cathode. Though minuscule, the electrical power produced by SMFCs has a number of potential applications such as sustaining low-power embedded electronics, pollutant remediation, or as a bio-sensing proxy for soil qualities and microbial activity. This discussion aims to emphasize the potential of SMFCs in addressing real-world environmental issues and to generate interest in the larger scientific community for broad interdisciplinary research efforts, particularly in field deployments. 

Biofilms are complex biomaterials comprising a well-organized network of microbial cells encased in self-produced extracellular polymeric substances (EPS). This paper presents a detailed account of the implementation of optical coherence elastography (OCE) measurements tailored for the elastic characterization of biofilms. OCE is a non-destructive optical technique that enables the local mapping of the microstructure, morphology, and viscoelastic properties of partially transparent soft materials with high spatial and temporal resolution. We provide a comprehensive guide detailing the essential procedures for the correct implementation of this technique, along with a methodology to estimate the bulk Young's modulus of granular biofilms from the collected measurements. These consist of the system setup, data acquisition, and postprocessing. In the discussion, we delve into the underlying physics of the sensors used in OCE and explore the fundamental limitations regarding the spatial and temporal scales of OCE measurements. We conclude with potential future directions for advancing the OCE technique to facilitate elastic measurements of environmental biofilms. 

Recovering nitrogen (N) from municipal wastewater is a promising approach to prevent nutrient pollution, reduce energy use, and transition toward a circular N bioeconomy, but remains a technologically challenging endeavor. Existing N recovery techniques are optimized for high-strength, low-volume wastewater. Therefore, developing methods to concentrate dilute N from mainstream wastewater will bridge the gap between existing technologies and practical implementation. The N-rich biopolymer cyanophycin is a promising candidate for N bioconcentration due to its pH-tunable solubility characteristics and potential for high levels of accumulation. However, the cyanophycin synthesis pathway is poorly explored in engineered microbiomes. In this study, we analyzed over 3,700 publicly available metagenome assembled genomes (MAGs) and found that the cyanophycin synthesis genecphAwas ubiquitous across common activated sludge bacteria. We found thatcphAwas present in common phosphorus accumulating organisms (PAO)Ca.‘Accumulibacter’ andTetrasphaera,suggesting potential for simultaneous N and P bioconcentration in the same organisms. Using metatranscriptomic data, we confirmed the expression ofcphAin lab-scale bioreactors enriched with PAO. Our findings suggest that cyanophycin synthesis is a ubiquitous metabolic activity in activated sludge microbiomes. The possibility of combined N and P bioconcentration could lower barriers to entry for N recovery, since P concentration by PAO is already a widespread biotechnology in municipal wastewater treatment. We anticipate this work to be a starting point for future evaluations of combined N and P bioaccumulation, with the ultimate goal of advancing widespread adoption of N recovery from municipal wastewater. 

Human-caused climate degradation and the explosion of electronic waste have pushed the computing community to explore fundamental alternatives to the current battery-powered, over-provisioned ubiquitous computing devices that need constant replacement and recharging. Soil Microbial Fuel Cells (SMFCs) offer promise as a renewable energy source that is biocompatible and viable in difficult environments where traditional batteries and solar panels fall short. However, SMFC development is in its infancy, and challenges like robustness to environmental factors and low power output stymie efforts to implement real-world applications in terrestrial environments. This work details a 2-year iterative process that uncovers barriers to practical SMFC design for powering electronics, which we address through a mechanistic understanding of SMFC theory from the literature. We present nine months of deployment data gathered from four SMFC experiments exploring cell geometries, resulting in an improved SMFC that generates power across a wider soil moisture range. From these experiments, we extracted key lessons and a testing framework, assessed SMFC's field performance, contextualized improvements with emerging and existing computing systems, and demonstrated the improved SMFC powering a wireless sensor for soil moisture and touch sensing. We contribute our data, methodology, and designs to establish the foundation for a sustainable, soil-powered future. 

The emergence of the Internet of Things and pervasive sensor networks have generated a surge of research in energy scavenging techniques. We know well that harvesting RF, solar, or kinetic energy enables the creation of battery-free devices that can be used where frequent battery changes or dedicated power lines are impractical. One unusual yet ubiquitous source of power is soil (earth itself) - or more accurately, bacterial communities in soil. Microbial fuel cells (MFCs) are electrochemical cells that harness the activities of microbes that naturally occur in soil, wetlands, and wastewater. MFCs have been a topic of research in environmental engineering and microbiology for decades, but are a relatively new topic in electronics design and research. Most low-power electronics have traditionally opted for batteries, RF energy, or solar cells. This is changing, however, as the limitations and costs of these energy sources hamper our ability to deploy useful systems that last for decades in challenging environments. If large-scale, long-term applications like underground infrastructure monitoring, smart farming, and sensing for conservation are to be possible, we must rethink the energy source. 

Abstract Hydrogel-encapsulated catalysts are an attractive tool for low-cost intensification of (bio)-processes. Polyvinyl alcohol-sodium alginate hydrogels crosslinked with boric acid and post-cured with sulfate (PVA-SA-BS) have been applied in bioproduction and water treatment processes, but the low pH required for crosslinking may negatively affect biocatalyst functionality. Here, we investigate how crosslinking pH (3, 4, and 5) and time (1, 2, and 8 h) affect the physicochemical, elastic, and process properties of PVA-SA-BS beads. Overall, bead properties were most affected by crosslinking pH. Beads produced at pH 3 and 4 were smaller and contained larger internal cavities, while optical coherence tomography suggested polymer cross-linking density was higher. Optical coherence elastography revealed PVA-SA-BS beads produced at pH 3 and 4 were stiffer than pH 5 beads. Dextran Blue release showed that pH 3-produced beads enabled higher diffusion rates and were more porous. Last, over a 28-day incubation, pH 3 and 4 beads lost more microspheres (as cell proxies) than beads produced at pH 5, while the latter released more polymer material. Overall, this study provides a path forward to tailor PVA-SA-BS hydrogel bead properties towards a broad range of applications, such as chemical, enzymatic, and microbially catalyzed (bio)-processes.